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AMP-activated protein kinase (AMPK) is a crucial cellular energy sensor. Once activated by falling energy status, it promotes ATP production by increasing the activity or expression of proteins involved in catabolism while conserving ATP by switching off biosynthetic pathways. AMPK also regulates me …
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Mitochondrial reactive oxygen species (ROS) production is a tightly regulated redox signal that transmits information from the organelle to the cell. Other mitochondrial signals, such as ATP, are sensed by enzymes, including the key metabolic sensor and regulator, AMP-activated protein kinase (AMPK). AMPK responds to the cellular ATP/AMP and ATP/ADP ratios by matching mitochondrial ATP ...
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Hardie D.G. Ross F.A. Hawley S.A. AMPK: a nutrient and energy sensor that maintains energy homeostasis. ... The genotyping primers used were P1 (tct cct tcc act ggg caa ctt cct g) and P2 (tgc tgt ccc cgg gac ct) for both the wild and the floxed type allele and P1 and P3 (ctg tgg tgc tct tgg ggg tg) for the KO allele, yielding 180-, 375 ...
6. Hardie, D.G. Molecular pathways: is AMPK a friend or a foe in cancer? Clin.Cancer Res.21, 3836–3840 (2015). 7. Eichner, L. J. et al. Genetic analysis reveals AMPKis required to support tumor growth in murine Kras-dependent lung cancermodels.Cell Metab. (2018). 8.
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