Toxicological screening - PubMed Central (PMC)
Toxicological screening methods and toxicological research on individual substances developed in the mid-1900s, and environmental toxicological studies developed in the mid-20 th century. The use of animals in toxicity studies began in 1920, when J. W. Trevan proposed the use of the 50% lethal dose (LD 50 ) test to determine the lethal dose of ...